Roi married Diane de Poitiers. Diane (daughter of Jean de Poitiers and Jeanne de Batarnay) was born 7 Aug 1503, Saint-Vallier, Drôme, France; died 22 Apr 1566, Anet, Eure-et-Loir, Centre-Val de Loire, France. [Group Sheet]

1. Marie-Diane Louise d'Angouleme de France was born 25 Jul 1538, Fossano, Cuneo, Piemonte, Italy; died 11 Jan 1618, Paris, Île-de-France, France.

Roi married Janet Stewart. Janet was born 17 Jul 1502, Edinburgh, Midlothian, Scotland; was christened 1502, Montrose, Forfarshire, Scotland; died 20 Feb 1562, London, Middlesex, England; was buried Feb 1562, Paris, Île-de-France, France. [Group Sheet]

1. Henry d'Angouleme was born 1551, Aix-la-Chapelle; died 1586, Aix-en-Provence.

Roi married Nicole de Savigny. Nicole was born About 1535, Lorraine, France; died 4 Mar 1590. [Group Sheet]

1. Henri de Saint-Rémy was born About 1557, Paris, Seine, Île-de-France, France; died 14 Feb 1621, Paris, Seine, Île-de-France, France; was buried , Paris, Seine, Île-de-France, France.

Roi married Catherine de Medici, Queen consort of France [Group Sheet]

1. Francis, II was born 19 Jan 1544, Château de Fontainebleau, Fontainebleau, Seine-et-Marne, France; was christened 10 Feb 1544, Fontainebleau, Seine-et-Marne, Île-de-France, France; died 5 Dec 1560, Orléans, Loiret, Centre-Val de Loire, France; was buried 23 Dec 1560, Saint-Denis, Seine-Saint-Denis, Île-de-France, France.